Two-wavelength quantitative phase unwrapping of dynamic biological processes

A two-wavelength phase unwrapping technique is implemented on two different phase microscopy systems developed in our laboratory: asynchronous and dynamic phase microscopes. Both systems are based on digital holography interferometric setups and in both systems one acquires two phase shifted interferograms to eliminate common phase noise. However, in the first system the two phase-shifted interferograms are separated in time, whereas in the second system they are separated in space. Asynchronous digital holography uses two near- simultaneous phase-shifted interferograms to recover phase information.