Comparison of the acoustic response of attached and unattached BiSphere™ microbubbles

Two systems that independently allow the investigation of the response of individual unattached and attached microbubbles have previously been described. Both offered methods of studying the acoustic response of single microbubbles in well defined acoustic fields. The aim of the work described here was to investigate the responses of single attached microbubbles for a range of acoustic pressures and to compare these to the backscatter from unattached single microbubbles subjected to the same acoustic fields.