Pyrolysis- GC/MS Analysis of Biomedical Components of the Pyrolyzate of Santalum Album Leaf Treated by Benzene/Ethanol Extraction

As the noble gardening tree growing specifically in China, Osmanthus fragrans is widely used to produce high-grade essence, special drugs and aromatic tea form its flowers. However, O. fragrans leaves are still underutilized and hence wasted. Therefore, in order to better utilize O. fragrans leaves and hence to expand its utility values and industrial scopes, we attempted to analyze the bioactive components, especially biomedical constituents from O. fragrans leaf by the way of Py-GC/MS (Pyrolysis- gas chromatograph/ mass spectrometer). After acetone extraction on O. fragrans fresh leaf by ultrasonic extraction for 5 h at 90degC, and with the removal of solvent, the extractive was pyrolyzed in 590degC He atmosphere, then the pyrolysis product was analyzed by online linked GC/MS. Relative content of each component was determined by area normalization, and 64 compounds representing 97.85% of the acetone ultrasonic extractive were identified from 65 peaks (Figure 1). The main constituents were as: friedelan-3-one (38.87%), lup-20(29)-en-3-one (12.46%), Stigmasterol,22,23- dihydro- (6.23%), Urs-12-ene (5.45%), benzoic acid,4-hydroxy-3- methoxy- (2.08%), stigmasterol (1.96%), cyclotetracosane (1.92%), acetone (1.59%), beta-amyrin (1.57%), benzoic acid,4- hydroxy- (1.32%), l,6-anhydro-.beta.-D-glucopyranose (1.32%), toluene,3-(2-cyano-2-phenylethenyl-) (1.22%), taraxasterol (1.20%), 1,2-propanediamine (1.07%), hexadecanoic acid (1.00%), ergosta-4,6,22-trien-3.alpha.-ol (1.00%), 1,2-benzenediol (0.91%), vitamin E (0.90%), campesterol (0.89%), etc. The results of function analyses suggested that the pyrolysis product from the acetone ultrasonic extractive of O. fragrans leaf is abundant in materials of noble natural medicines, and also contains constituents which can be developed into top value- added materials of cosmetics, biofuel and industrial. This is the first report here that showed underutilized leaf of O. fragrans contains abundant biomedical constituents and can be developed into top value-added products.