Biochemical-Powered Pump with an Asymmetric Enzyme Membrane

A biochemical-powered pump was constructed by using two funnel type glass tubes separated by an enzyme diaphragm membrane, in which catalase was immobilized onto the single side of the dialysis membrane. By applying hydrogen peroxide (H
₂
O
₂
) solution into the non-enzyme side of the glass tube, the tube pressure at the enzyme side increased continuously. In consequence, an active transportation of H
₂
O
₂
through the asymmetric enzyme membrane could induced the pressure increase in the enzyme side tube (max.: 5000 Pa), thus resulting in the discharge of buffer solution from the enzyme-side tube with non-pulsating flow.