Analysis of Micrometer Sample Flows in a Non Coaxial Sheath Flow Device

In this paper we present a novel non coaxial sheath flow device with channel dimensions of 50 μm in square at the focusing region. Analyses of the adaptable sample flow are carried out where the focusing limitation and its profile variations are shown using confocal microscope measurements. A comparison of the sample flow height inside the channel and its relative fluorescence intensity is highlighted. Additionally, the influence of the hydrodynamic focusing on the sample profile and its uniformity over a certain distance is shown.