A Robust Fluorescence Multiplex Immunoassay Biosensor Designed for Field Applications

A novel portable fluorescence-based biosensor designed for field applications has been developed and successfully applied to the determination of two analytes, a neuropeptide model Substance P, and a small toxin Aflatoxin BI. A competitive immunoassay format called SPIT-FRI for Solid-Phase Immobilized Tripod for Fluorescent Renewable Immunoassay is used. The simplicity and robustness of the system apparatus is shown. Results clearly illustrate the assets of the developed array biosensor, by allowing in a portable instrument (i) simple regeneration steps, (ii) real-time monitoring, and (iii) multiplex detection thanks to a localized and cumulative fluorescence measurement.