Biosensor implementation for cardiovascular risk marker analysis

In this paper we presented the detection of cardiovascular marker protein set in the form of sandwiched- antibody microarrays. The antibody set consist of C-reactive protein, fatty acid binding protein, myoglobin, D-dimer, troponin T protein, known cardiovascular risk markers in human serum. Chemically-modified glass substrate surfaces were used as solid support for immobilization of sandwiched, antibody-antigen-antibody structures. An optical scanner system was used for the detection of fluorescent-labeled, captured antibody set. We have shown that the proposed structure/process is capable of detecting 1/spl mu/g/ml concentration of antigens, used in this study.