An ultra-high sensitive protein lab-on-a-chip on polymer for prostate cancer diagnostics

In this paper, we report a disposable, fast and high sensitive protein lab-on-a-chip on cyclic olefin copolymer (COC) substrate, which can be used for the detection of prostate specific antigen (PSA), a biomarker of prostate cancer. In this protein lab-on-a-chip, chemiluminescence-based sandwich immunoassay was developed for detecting PSA with the detection limit of 1 ng/mL. With the developed immunoassay, the protein lab-on-a-chips on polymer have successfully detected PSA in the range of 4-200 ng/mL. The protein lab-on-a-chip developed in this work can also be used for the detection of other biomarkers and proteins.