Quantitative analysis of volumetric TPLSM data

This paper describes initial algorithm development in support of research on a new all-optical histology technique based on two-photon laser scanning microscopy (TPLSM) and laser tissue ablation. This new imaging modality makes it possible to obtain quantitative information that can be used to measure physiological parameters of interest. We present our initial image processing approach and volumetric rendering results. Two examples of useful quantitative parameters are presented: measurement of blood vessel volume fraction and cell counting.