The effect of copper ion on blood coagulation

The effect of copper ions on the blood coagulation time was determined. In the experiment that was performed in vitro, there were three different aliquots for each blood sample. The three aliquots were composed of the control, 0.05 mM CuSO/sub 4/, and 0.1 mM CuSO/sub 4/. Thirty two microliters of 0.1 M CaCl/sub 2/ was then added to each aliquot to initiate blood coagulation. When the copper ion concentration was increased, there was a significant increase in blood coagulation time.