Research on High-Precision Embryo Electroactivation Chip Based on Electric Field Optimization and Calcium Oscillation Regulation

In embryo engineering, the activation of early embryos is a critical step to initiate development, and the activation effect directly influences subsequent development. Addressing issues in traditional electroactivation devices, such as unstable activation effects, low cell survival rates, uneven electric field distribution, and electrical damage, this study constructs an oocyte electroactivation model and systematically investigates the effects of microelectrode shape and electrical pulse parameters (including pulse voltage, pulse width, cell size, and solution conductivity) on electroactivation. The results show that arc-shaped microelectrodes can generate a stable and uniform electric field at low voltages, reducing local overvoltage. Among the parameters, pulse voltage and solution conductivity are key factors determining electroactivation effectiveness, while pulse width and cell size have relatively minor effects. Furthermore, this study innovatively designs and manufactures a high-precision arcshaped microelectrode electroactivation device. Experimental results show a 9.4% increase in blastocyst formation rate compared to conventional electroactivation systems, validating the effectiveness of the research. This study provides optimization directions for research in the field of embryo engineering.