Fiber-Based OCT Guided Subretinal Injection in Ex-Vivo Mice Eyes

We present the use of a fiber-based Optical Coherence Tomography (OCT) system for precise targeting of the subretinal space in subretinal injection (SI). The system integrates OCT probes with a 33G needle, enabling real-time visualization of the retinal layers during SI in ex-vivo mice eyes. The setup includes a micromanipulator, an injection pump, and a software interface for user-friendly operation. This OCT-controlled system aims to standardize SI in mice, facilitating accurate preclinical validation of retinal gene therapies.