Hyperspectral Phase Imaging via Optical Differential Microscopy

Edge detection is critical for emerging image processing technologies. Here, we present a dual-channel optical differential system that faithfully retrieves full edges of two-dimensional (2D) images. The system, leveraging the modified Wollaston prism, is compatible with hyperspectral imaging (HSI), enabling real-time phase imaging across 450-750 nm with 30 spectral channels. Experimental results demonstrate that the peak signal-to-noise ratio (PSNR) of the image is improved over 6 dB with the proposed system. Meanwhile, it indicates that edge imaging can achieve higher contrast on biological samples. This work demonstrates the system’s potential for wide applications like target recognition and image enhancement, laying the foundation for hyperspectral phase edge microscopy.