Correlation Between Oxygen Transfer Coefficient and Operational Parameters in Circulatory Cell Culture System

The fed-batch culture system offers the advantage of maintaining high cell growth rates and metabolic activity in cell cultures, resulting in high cell density and product concentration. However, the oxygen transfer process within this system has not been fully characterized. This study aims to investigate the relationship between the oxygen transfer coefficient (kLa) and the operational parameters of the system, and to develop a predictive model for kia based on dimensionless analysis. Experiments were conducted using an intelligent recirculating culture device, varying parameters such as impeller speed, liquid volume, oxygen volume fraction, internal pressure, peristaltic pump speed, and concentration gradient. The results indicate that the model can accurately predict kia in the recirculating culture system, with a coefficient of determination (R2) of 0.9876 and a root mean square error (RMSE) of 6.123456 x 10-⁷• This study offers theoretical support and practical guidance for the design, optimization, and control of bioreactor systems.