Integrated Microfluidic Rapid Response and Highly Sensitive Electrochemical Aptasensor for Simultaneous Detection of Aflatoxin B1 and Deoxynivalenol

A novel electrochemical aptasensor was designed for the simultaneous detection of aflatoxin B1 (AFB1) and deoxynivalenol (DON) using dual-working microelectrodes and PDMS-based microfluidic channels. The system provides high sensitivity, with detection limits of 7.74 pg/mL for AFB1 and 0.172 ng/mL for DON across broad ranges (0.03 − 300 ng/mL for AFB1, 0.3 − 3000 ng/mL for DON). Results can be obtained within 20 minutes, and the platform exhibits excellent reproducibility, selectivity, and stability. Validation with corn extract highlights its potential for on-site food safety monitoring.