Analysis of DNA Sequences from Human Sweat and Comparison with Blood Sample

Genome studies heavily rely on valuable sources of nucleic acids found in biological fluids. Sweat, an easily collectible and cost-effective biofluid, has emerged as a promising material for genomic research. However, the suitability of sweat DNA for genome sequencing compared to venous blood DNA requires further investigation. Sweat samples were collected from four individuals, and a statistical comparison was conducted between sweat DNA and healthy blood DNA reads. The study focused on four key aspects: genome coverage, fragment lengths, mapping quality, telomere sequences, and chaos game representation (CGR) analysis. The length, quality, and genome coverage of all DNA fragments were examined, and tandem repeats in the telomere sequences of the study samples were identified in this article. The statistical analysis revealed significant similarities in genomic study outcomes between sweat and blood samples. The results suggest that sweat has the potential to be an alternative substitute for genomic analysis, offering a cost-effective and easily accessible avenue for future investigations. Our study highlights the potential utility of sweat as a valuable source of nucleic acids for genomic research. While significant similarities were observed between sweat and blood samples regarding genomic outcomes, further studies are warranted to explore the full capabilities of sweat DNA in genome sequencing. Using sweat in genome research could lead to cost-effective and accessible approaches for future genomic investigations.