Design and development of a modular perfusion bioreactor prototype using 3D printing

This study outlines the conceptual and design framework for a modular bioreactor intended to advance accessibility in tissue engineering by providing an optimal environment for three-dimensional cell growth and differentiation. By integrating 3D printing technology, we propose an innovative and cost-effective approach to bioreactor fabrication streamline the development and customization of tissue engineering applications. While the actual cell cultivation within the bioreactor has yet to be undertaken, preliminary analyses—including rigorous rheological evaluation and computational simulations—validate the bioreactor’s potential to maintain a uniform, contamination-free culture environment. This research sets the stage for future experimental work, aiming to validate the bioreactor’s effectiveness in supporting cell development and specialization. The anticipated outcomes promise to mark a significant step toward the clinical translation of bone tissue engineering solutions, embodying a new horizon in regenerative medicine.