Gold/Cerium(IV) Oxide-Modified Flexible Electrodes for Enzymatic Detection of Triglyceride

Triglyceride (TG) testing aids in determining the likelihood of heart disease development. An elevated TG level increases the risk of heart attack and stroke by causing atherosclerosis. Point-of-care TG detection not only identifies these risks earlier but also enables user-friendly, cost-effective onsite usage. Thus, in this work, for the detection of TG, an electrochemical biosensor has been developed with a primary focus on portability and affordability. The sensor consists of an electrochemical cell fabricated with screen-printed carbon electrodes printed on commercially available overhead projector sheets. The detection approach is enzymatic, with lipase acting as the primary detector, immobilized on an Au/CeO²-modified working electrode. The current generated during the enzymatic reaction between the immobilized lipase and TG sample is proportional to the sample concentration. The electrochemical technique, cyclic voltammetry (CV), is used to quantify the amount of TG. The sensor is fabricated and tested with a solution of glyceryl tributyrate at a concentration range of 0.2 to 10 mM without the need for sample pretreatment. A portable potentiostat is utilized to perform the electrochemical characterization of the fabricated device. The calculated limit of detection and limit of quantification of TG are 0.25 and 0.76 mM, respectively. The proposed design is compact, easy to fabricate, and highly portable, showing great potential for continuous point-of-care monitoring of human TG levels.