Estimation of the contribution of all-trans and cis-isomers to carotenoid absorption

We propose an original approach to determine the concentration of cis-isomers of carotenoids in solvents by performing the fitting of experimental data with the help of differential evolution. The total contribution of cis-isomers to the resulting optical response was modelled by a Gaussian curve, whereas the spectrum of the all-trans isomer was calculated within the framework of semi-classical theory.