Lateral Flow Assay Dynamics Monitoring with Real-Time Optical and Magnetic Registration: Rapid and Quantitative Tumor Marker Detection

This study introduces an innovative biosensor approach for point-of-care molecular biomarker detection utilizing lateral flow assay dynamics monitoring with real-time optical and magnetic registration. Spectral interferometry was employed to analyze antibody kinetics targeting the tumor marker CYFRA21-1, leading to the development of rapid quantitative lateral flow tests. Achieving a broad dynamic range and rapid biomarker detection is demonstrated. Furthermore, a novel tool for controlling and eliminating errors in label quantities is proposed, enhancing the robustness of quantitative systems in real-time dynamics-based approaches.