New mechanisms in stem cells differentiation and tissue regeneration discovered by optical imaging

Using FLIM and multiphoton fluorescence microscopy we investigated the metabolic status in mesenchymal stem cells during adipogenic, osteogenic and chondrogenic differentiation, the metabolic activity and intracellular pH in iPSC differentiating in dermal, epidermal, neuronal directions, in 3D neurospheres from iPSCs, in neural spheroids with Down syndrome, metabolic changes in living islets of Langerhans, and during liver regeneration.