Revolutionizing Live-Cell Tracking with Machine Learning: Bridging the Gap Between Automation and Precision in Quantitative Biology

In the rapidly evolving field of quantitative biology, researchers are increasingly reliant on precise and quantitative analysis tools to gain deeper insights into complex biological systems. Among these tools, quantitative image analysis has gained prominence, empowering cell biologists to quantitatively characterize various aspects of cellular processes. One particularly informative yet challenging aspect of this research is live-cell tracking, which enables the dynamic monitoring of molecules and entities of interest within living cells. While existing live-cell tracking methods have provided valuable insights, many of them are semi-automatic, requiring manual parameter adjustments and human supervision. In response to this challenge, this project embarks on a journey to bridge the gap between the complexity of live-cell tracking and the need for automation and accuracy. Our primary objective is to integrate machine learning techniques into the live-cell tracking process, aiming to enhance its efficiency and reliability.This project recognizes the critical role of live-cell tracking in unraveling the mysteries of cellular behavior and molecular dynamics. By harnessing the capabilities of machine learning, we aspire to revolutionize the tracking process, making it more autonomous and less reliant on manual intervention. The anticipated outcomes of this endeavor extend beyond the realm of live-cell tracking, promising to empower cell biologists and researchers in their pursuit of comprehensive, quantitative insights into the intricacies of biological systems. As of late, specialists have concocted different novel thoughts regarding how to further develop the following calculation. The technique to increase the accuracy of direction and speed of the cells has been raised. Both the appearance highlights that anticipate that the condition of a particular cell changes slightly between back-to-back outlines and this expectation have been raised. It should go without saying that more parts need to be combined to more closely follow cells. Here, we are contemplating carrying out AI calculation to 1) figure out which element of the cells loads more with regards to cell following. 2) whether it’s feasible to think of speculation of following technique by carrying out AI. 3) ideally, to sum up a decent calculation for live-cell following and coordinate straightforwardly into the picture examination pipeline in our lab.