SARS-CoV-2 Infection Caused a Damage on Vascular Bed Co-Cultured with Bronchial Organoids in Microfluidic Device

This paper reports the effect of SARS-CoV-2 infection on bronchial organoids and endothelial vasculature formed with human primary cells in microfluidic devices. Established model enables us to analyze virus infection site and interaction on multiple type of cells, showing infection tropism to bronchial organoids and vasculature damages. Further analysis revealed that vascular structure was damaged through paracrine pathway caused by cytokines released from infected bronchial organoids. These results demonstrated that proposing model is capable to evaluate complex mechanism of vascular symptoms in COVID-19 and comparison of pathogenicity between multiple strains.