Micro Plasma Gene Transfection System for Uniform Expression

We developed a micro gene transfection system for creating large amounts of cells with the same gene expression profile. For introducing the same quantities of genes into each cell, making same gene transfer path on its cell membrane is a first step. To make the path, non-thermal atmospheric pressure plasma was used. Using micro-through-hole device as a micro-skimmer for localized plasma exposure, plasma exposure area was defined on cell membrane. At the cell membrane exposed with plasma, permeability was improved, and gene-coding plasmids were effectively transferred inside cells. Our microdevice will enable creating cells with uniform quality.