Formation Dynamics of DNA Condensates in Monodisperse GUVs Toward Construction of Artificial cells with Nucleus

There has been an effort to construct hierarchical molecular structures that mimic living cells. For this purpose, a microfluidic system to generate monodisperse giant unilamellar vesicles (GUVs) is expected to be a standardized platform. Here, we studied the dynamics of DNA condensate formation, as a mimic of the nucleus, in monodisperse GUVs generated by our microfluidic system. Encapsulated DNA nanostars undergo liquid-liquid phase separation within GUVs in a sequence-dependent manner. We clarified the dependence on time and sticky end (SE) length in addition to the concentration control of components such as DNA oligomers and salt.