High-Throughput Generation of Giant Liposomes Utilizing Step Emulsification and Parallelized Droplet Transfer Channels

We propose a novel droplet microfluidic device for high-throughput generation of giant liposomes without surface modification of microchannel by utilizing the step emulsification and the parallelized droplet transfer microchannels. Unlike the conventional microfluidic techniques for production of giant liposomes, limiting the throughput due to the low production rate of droplets in single microfluidic droplet generator, the proposed device could produce giant liposomes with high throughput manner. Our microfluidic device consists of two main parts; (i) water-in-oil (W/O) droplet generation through 100 parallelized micro nozzles, and (ii) generation of uniform W/O/W droplet through parallelized droplet transfer channels. After generation of W/O/W droplets at 332 Hz, they transformed into giant liposome by dewtting process.