An SPR Microfluidic Devices for Identification of Human Herpesvirus 4 And SARS-Cov-2

Recent studies reveal that the severity of COVID-19 may be correlated with the presence of HHV-4. Although numerous devices can detect HHV-4 or SARS-CoV-2, to our knowledge, no device has been used to detect both simultaneously.Herein, we design a device that can perform a duplex polymerase chain reaction (PCR) in a microfluidic channel and detect amplicons through a surface plasmon resonance (SPR) sensor. This device can reduce the required PCR time by 55% compared to traditional instruments while yielding a similar amount of amplicon. Moreover, the device can detect desired genes by extracting cells infected with HHV-4/SARS-CoV-2.