Electrophysiological Characterizaton Of A Novel, Transwell-Transferred, Human Neural Nociceptive Microphysiological Circuit Atop Polymer/Steel 3d Microelectrode Arrays

Accelerated therapeutic compound screening has become of paramount importance in current decades to combat both recent drug crises (e.g., opioid epidemic) prevalent in society and advance drug screening. To this end, BioMEMS-enabled in vitro models are of great interest, as means to replicate relevant physiology in a laboratory setting. This work reports the comparative electrophysiological response from a first-of-its-kind, in vitro, human peripheral to central nervous system (PNS-CNS), nociceptive organoid-based Microphysiological System (MPS) construct from a custom, Polydimethylsiloxane (PDMS)-insulated, 3D Microelectrode Array (3D MEA). The microfabrication and impedimetric characterization of such a polymer/stainless steel (SS) 3D MEA, is additionally reported, as well as an MPS transwell-transfer method for inhibitory drug mediated bioelectronic interrogation.