The effect of cell culture alkalinity caused by positive electrostatic charge therapy on breast cancer cells apoptosis

Breast cancer and consequent tumor metastases are almost hard challenges for human health in the last years. The tumor metastases affect treatments adversely. Positive Electrostatic Charge Therapy (PECT) is a new and different method to treat malignant tumors. The evaluated two breast cancer cell lines (MCF-7 and MDA-MB-231) indicated that despite MCF-7 and normal cells, PECT induced significant apoptosis in MDA-MB-231. Positive charge accumulation likely repels cations and the proton is an important cation that affects intracellular pH. In this study, we investigated the effect of alkaline cell culture on Benign and malignant breast cancer cell apoptosis. MCF-7 and MDA-MB-231 cell lines were incubated in alkaline culture with a pH of 8.8. The cell lines’ apoptosis was monitored through microscopy and flow cytometry methods. The results showed more apoptosis in MDA-MB-231 than MCF-7 as expected. The obtained result could be due to the higher culture acidity of MDA-MB-231 than MCF-7. The results can suggest new methods for treating malignant breast cancer in the future.