Weakly-Supervised Cross-Domain Segmentation of Electron Microscopy with Sparse Point Annotation

Accurate segmentation of organelle instances from electron microscopy (EM) images plays an essential role in many neuroscience researches. However, practical scenarios usually suffer from high annotation costs, label scarcity, and large domain diversity. While unsupervised domain adaptation (UDA) that assumes no annotation effort on the target data is promising to alleviate these challenges, its performance on complicated segmentation tasks is still far from practical usage. To address these issues, we investigate a highly annotation-efficient weak supervision, which assumes only sparse center-points on a small subset of object instances in the target training images. To achieve accurate segmentation with partial point annotations, we introduce instance counting and center detection as auxiliary tasks and design a multitask learning framework to leverage correlations among the counting, detection, and segmentation, which are all tasks with partial or no supervision. Building upon the different domain-invariances of the three tasks, we enforce counting estimation with a novel soft consistency loss as a global prior for center detection, which further guides the per-pixel segmentation. To further compensate for annotation sparsity, we develop a cross-position cut-and-paste for label augmentation and an entropy-based pseudo-label selection. The experimental results highlight that, by simply using extremely weak annotation, e.g., 15% sparse points, for model training, the proposed model is capable of significantly outperforming UDA methods and produces comparable performance as the supervised counterpart. The high robustness of our model shown in the validations and the low requirement of expert knowledge for sparse point annotation further improve the potential application value of our model.