Single-Cellular Dynamic Mechanical Analysis of Live 3d Organoids Under Light-Sheet Fluorescence Microscopy

We have developed a novel microscopic analysis system that combines the functions of light-sheet fluorescence microscopy (LSM) and dynamic mechanical analysis (DMA). We have integrated the three uniquely designed components of (i) a MEMS dynamic compression device with a μ-force sensor, (ii) a high-speed 3D light-sheet scanner and an imager, and (iii) a custom-programmed image-based 3D modeling algorithm. Here, we demonstrate spatially-resolved mechanical characterization of viscoelastic materials under high-resolution 3D fluorescence microscopy for the first time.