Study of an unsteady amperometric biosensor using a computational model

Biosensors belong to a category of sensors designed for measuring organic substances. These sensors offer numerous benefits for utilization, encompassing equipment miniaturization and portability, thereby facilitating easy deployment in diverse locations. The main goal to use a device such as a biosensor are its benefits combined with its fast methodology, therefor ways of make it became safety faster a desirable and crucial, and a possibility to use its readings even in a unsteady state with a previous settle time laps may be useful to an even faster methodology. The objective of this study is to assess the operation of an unsteady monolayer enzymatic amperometric biosensor through the utilization of mathematical modeling. The working principle of the biosensor is examined using Partial Differential Equations (PDEs) and the corresponding boundary/initial conditions that govern and define this type of device. The system equations are solved using the Finite Difference Method (FDM) techniques, aided by computer code to obtain the solution for the analyzed system. Subsequently, simulations are conducted to evaluate the influences of various factors on the system’s response. Sensitivity tests are performed on each parameter of the monolayer enzymatic amperometric biosensor. The outcomes reveal significant impacts of factors such as the maximum enzymatic rate (V
MAX
), enzymatic layer thickness (d), and initial substrate concentration (S
0
) on the system’s response, which are considered vital in biosensor projects.