Electrical tests for acetylcholinesterase and activation with CD3 (antigen) and anti- CD3 (antibody) with proteins

We present the manufacturing of a microfluidic-electrochemical biosensor with interdigitated electrodes. We performed electrical tests for three different samples containing acetylcholinesterase, anti-CD3 and CD3 to identify the protein concentration. We established that the current is measured in relation to the reaction time at a fixed potential, which allows the detection of protein concentration at 4 mM and 10 mM. In particular the methods of using these proteins and compounds, preferably antibodies, which bind or modulate the activities of these proteins, are used to treat cancer, allergies, infections and inflammatory conditions. Electrochemical biosensors have become victorious candidates for medical development due to the ease of turning a biological interaction into an electrical signal. The aim of this work is to use the biosensor to detect the concentration of proteins and antigens that have anti-inflammatory action. Electrical tests show that the current increases with increasing protein concentration. The electrical tests were performed by KEITHLEY current-voltage and time analyser.