Simulation and Intelligent Data Mining of Molecular Transport Through Multiple Nanopores in an Electroporated Giant Unilamellar Vesicle

Microfluidic electroporation, or electro-permeabilization is the short, high-voltage pulses produced by an external electric field on a non-electrolytic cell to study intramolecular organisms. This is a sophisticated and newly established substantial molecular transport technique for gene transfection, cancer and tumor treatment, transdermal drug delivery, and so on. In this study, a dysregulated cell is used to simulate the molecular transport from the exterior of the cell to the interior of the cell and analyze the molecular transport with respect to time for direct clinical use. We have developed a non-electrolytic microelectroporation electrode surface, on which metal electrodes are coated with a dielectric. A COMSOL-based numerical analysis was used to calculate the molecular transport and dielectric material properties dependent electric field produced in the dielectric, fluid flow, electroporation, or electro-permeabilization field and Clausius-Mossotti factor for a
$10.008\ \mu \mathrm{m}$
diameter giant unilamellar vesicle (GUV). The molecular transport from the exterior of the giant unilamellar vesicle to the interior has been calculated for the different points situated in and around the giant unilamellar vesicle. The molecular transport is calculated for several different points, chosen carefully from an infinite number of points around the total system. They are analyzed depending on the different purposes and uses that can directly be used for clinical purposes.