Integrating Live-Cell Imaging with Magnetic Manipulation System

The internalization of magnetic nanorobots within live cells provides insights into their behaviour under the influence of an external magnetic field. However, the heat generated by the Helmholtz coil poses a significant challenge, leading to cell death. Most commercially available systems rely on metallic components to facilitate heat transfer. However, if these systems are placed inside the triaxial Helmholtz coil of the experimental setup, the following magnetic field will create eddy currents, leading to unintended heating consequences. Additionally, the limited space inside the Helmholtz coil presents a significant constraint for accommodating commercial systems. Integrating standard commercial live cell setups with magnetic propulsion setups, typically composed of triaxial Helmholtz, poses challenges. Additionally, thermal control is crucial as temperature maintenance within a degree is necessary, despite intermittent heat loads generated by coil drives with an extensive dynamic range. To address these issues, we have constructed and operated a system that successfully overcomes these challenges. A detailed account of the system's performance is given in this manuscript. The information presented herein will facilitate comprehensive biophysics studies. In this research, we present a unique setup that ensures precise temperature and pH control to enable extended viability of live cancer cells, facilitating in-depth studies of magnetic nanorobot behaviour within the cells subjected to an external magnetic field. The study of magnetic nanorobots within live cancer cells holds promise for advancing targeted therapies. However, maintaining appropriate temperature and pH conditions is critical for long-term cell viability. This research aims to overcome temperature fluctuations induced by the external magnetic field and incompatibility issues with commercially available systems by developing an integrated setup for optimal temperature and pH control. This innovative setup enables prolonged cell viability, facilitating in-depth investigations and advancing our understanding of magnetic nanorobot-cell interactions.