Surface Imprinted Electroimpedance Biosensor for Detecting α-Synuclein for Parkinson's Disease

Surface Imprinted Polymers (SIP) are a unique category of molecular imprinted polymers that do not require specific chemical reactions to create the nanoimprint desired for detecting target analytes with high specificity. In this work, we demonstrate a soft-printed and low-temperature processed SIP bio-recognition nanomaterial for label-free detection of high impact analyte, α-Synuclein (αSyn), using electroimpedance spectroscopy. αSyn, a target selected due to its viability for monitoring early onset and progression of the Parkinson's Disease (PD), was detected over a broad concentration range of 10 fg/L to 10 μg/L. The high specificity of the biosensor was demonstrated by analyzing a biomolecule, β-Synuclein, which is highly homologous to αSyn, but is not a factor towards PD. The simplistic, soft-printed SIP EIS biosensor is a label-free, and specific method for quantifying αSyn over broad concentration range that could revolutionize PD testing in the future.