Investigation on Cell Orientation Induced by Various Femtosecond Laser Ablated Microstructures

Femtosecond laser ablation technology has been widely used in the past decade for its high precision and flexible processing capabilities in 3D micro/nano machining. This study aims to explore the application of this technology to fabricate microstructures capable of inducing cell orientation in vitro. Three different microstructures were fabricated by varying the frequency of laser pulses and implementing a moving platform that controls the motion of substrates. The results demonstrate that the stripe array structures specifically showed excellent ability in inducing cell orientation and controlling cell growth along the striation structure. This proposed method exhibits major potential as a simple yet powerful tool for investigating cellular responses to external mechanical stimuli.