Bi-directional Feature Fusion Generative Adversarial Network for Ultra-high Resolution Pathological Image Virtual Re-staining

The cost of pathological examination makes virtual restaining of pathological images meaningful. However, due to the ultra-high resolution of pathological images, traditional virtual restaining methods have to divide a WSI image into patches for model training and inference. Such a limitation leads to the lack of global information, resulting in observable differences in color, brightness and contrast when the re-stained patches are merged to generate an image of larger size. We summarize this issue as the square effect. Some existing methods try to solve this issue through overlapping between patches or simple postprocessing. But the former one is not that effective, while the latter one requires carefully tuning. In order to eliminate the square effect, we design a bidirectional feature fusion generative adversarial network (BFF-GAN) with a global branch and a local branch. It learns the inter-patch connections through the fusion of global and local features plus patch-wise attention. We perform experiments on both the private dataset RCC and the public dataset ANHIR. The results show that our model achieves competitive performance and is able to generate extremely real images that are deceptive even for experienced pathologists, which means it is of great clinical significance.