Development of highly sensitive and stable electrochemical sensor for H2O2 detection by facile synthesized copper nanoparticle thin film

Copper nanoparticles are used in several areas related to nanotechnology, including biomedicine, pharmaceuticals, bioremediation, molecular biology, bioengineering, genetic engineering, colour degradation, catalysis, cosmetics, and textiles. The structural features and biological impacts of copper nanoparticles show promise in the realm of life sciences, particularly in the development of biosensors. In this study, copper nanoparticles (Cu NPs) were synthesized, characterized, and used to modify glassy electrodes for the electrochemical analysis of hydrogen peroxide. The copper nanoparticles were prepared using a chemical reduction process and analyzed using SEM, XRD, TEM, and AFM. Using the spin coating process, the synthesized nanoparticles were used to create a thin film electrode. The CV Studies (CV Curve, Current VS Hydrogen peroxide) demonstrate that the Cu NP electrode is effective for the selective determination of hydrogen peroxide even in the presence of ascorbic and uric acid. The observed linear range of Cu NP for hydrogen peroxide was 0.5M to 2.5M, with a detection limit of 3.94M. It was established that newly developed thin film copper nanoparticles are suitable for non-enzymatic hydrogen peroxide measurement. As a result, it has the potential to be used for detection in real-life samples such as human blood serum.