Accurate gravimetric calibration of micro-pipettes using double evaporation trap with temperature correction and uncertainty budget

In disciplines including medicine, chemistry, biology, pharmacy, and genetics, volume measurements are most effectively performed using micropipettes. In order to calibrate micropipettes correctly and calculate uncertainties, laboratories must guarantee that the data produced by this equipment are accurate. The goal of this work is to outline the ideal procedure for calibrating micropipettes, under the specified measurement conditions, down to 1 microliter with sources of errors to be addressed, studied and corrected. Also described is a model for calculating measurement uncertainty. Uncertainty level of 0.02 μL in the calibration level of 1 μL could be achieved.