Evidence for the Successful Manipulation of a Magnetic Nanorobot Inside the Hard Tumor of a Live Animal

Observation of nano/micron-scale objects inside living tissue is a significant challenge in all nanoparticle drug delivery research. Helical Magnetic Nanorobots[1] (HMNs) also face this problem, where any translation due to a rotating magnetic field is not readily observable. The problem arises due to the scattering nature of living tissue. Here we demonstrate a new technique to determine if HMNs have achieved translation by post-experimental analysis of tissue. We use non-magnetic tracer particles to identify the site of injection. The diffusive spread of HMNs is minimal due to the rigidity of the tissue. By observing the microtome slices of the tissue, we could observe HMNs far from their injection site, which would be possible only due to magnetic field-induced translation. This allows us to calculate the pitch of HMNs inside a living tissue to be about 20–25 nm.