Metrological analysis of the optical response to be acquired from an oligonucleotide library

The subject of this paper relates to the measurable effects of light behavior in biological materials. The molecular complementarity of the two strands of the DNA double helix makes it possible to model changes in DNA sequences manifesting by related changes in their melting temperature, thermodynamic data and optical properties. This contribution is focused on the metrological problems connected with evaluation of parameters specifying the response of a virtual integrated oligonucleotide library. The presented analysis is concerned with the fluorescence signals to be obtained as a result of hybridization and detected by a CCD camera. Improving processing of acquired images is considering taking into account image smear that is still a fundamental limitation of the CCD detecting technique.