Antifouling for Electrochemically Biosensing in Body Fluids

This paper presents a simple and robust antifouling sensor based on a nano-wrinkle electrode with bovine serum albumin for electrochemically detecting small molecules in complex body fluids. The nano-wrinkle was prepared by a one-step shrinking technique, being capable of excluding large proteins in physical structures and enhancing the response of small molecules. The prepared sensor demonstrates excellent antifouling capability under discontinuous or continuous exposure to proteins or fetal calf serum, which preserves 97% of its original signal after a 21-day exposure to fetal calf serum and enabled the quantification of dopamine in the protein-coexisted environment with a low limit of detection (LOD) (1.09 μM) and a wide detection range from 20 to 1105 μM.