Measurement of intracellular electrical activity from two smooth muscle layers

An apparatus based on the sucrose gap technique is presented, and its use for simultaneous measurements of contractile and intracellular electrical activity from the two smooth muscle layers of the colon is discussed. An L-shaped muscle preparation consisting of a leg from the circular muscle layer and a leg from the longitudinal muscle layer is used. A theoretical discussion of the device's operation is presented. Experimental results that validate the theory are included.<>