Transcutaneous measurement of bio-substances by suction effusion fluid from skin

Successful transcutaneous blood glucose monitoring on human subjects has been achieved. Effusion fluid is collected from the skin using a recently developed suction apparatus, and glucose concentration in the fluid is measured. During a 75-g oral glucose tolerance test on normal subjects, frequent transcutaneous monitoring of blood glucose change was achieved with an improved ion-sensitive field effect transistor glucose sensor which can measure glucose concentration in only 5- mu l samples.<>