Synthesis of Silver Nanoparticles from Lemongrass Leaves Induced Wound Healing by Reduction ROS Fibroblasts

A wound is damaged tissue caused by injury or disease. Meanwhile, fibroblasts are cells found in connective tissues that synthesize, deposit, and remodel the extracellular matrix during wound healing and the imbalance between the prooxidatives and antioxidants, which contribute to increased reactive oxygen species (ROS). Generally, increased ROS production leads to progressive oxidative damage that slows healing and causes cell death. However, the synthesis of silver nanoparticles with Cymbopogon citratus (Lemongrass) extract (NPsCc) decreases ROS production. Therefore, the purpose of this study was to observe the effect of silver nanoparticles extracted from C. citratus on ROS fibroblast production. The study was divided into ten groups, namely without treatment, ascorbic acid, hydrogen peroxide, C. citratus leaves extract, and five groups of silver nanoparticles with C. citratus (NPsCc) concentrations of 40 ppm, 20 ppm, 10 ppm, 5 ppm, and 2.5 ppm. Subsequently, the results showed that the 10 ppm NPsCc group decreased ROS fibroblast production compared to the negative control and other NPsCc groups. This was indicated by the percentage of cells with low green fluorescence cell intensity of 48.37%.