Associating vascular imaging with hypoxia and cell survival in vivo for Biophotonics applications

As oncological treatments aim to move towards personalize medicine, increase tissue response monitoring has become the focus of research. In particular, spatially resolved in vivo information of hypoxia and its relationship to cell survival is required to maximize the patient's benefit. At the same time, several preclinical imaging approaches were developed in the past years, including Photoacoustic, Bioluminescence imaging (BLI) to quantify blood flow/volume. Here Photo acoustic Imaging (PAl) is used to study tumor hypoxia for Photodynamic Therapy (PDT) monitoring to predict cell survival. The Photoacoustic and Bioluminescence signals' temporal dynamic are used to detect vascular changes during and post-PDT.