Synthesis of Zinc Oxide Nanoparticles and Their Antibacterial Activity

Zinc oxide nanoparticles (ZnO NPs) have received significant interest in bioengineering. Herein, ZnO NPs synthesis was performed via sol-gel method using precursors including zinc acetate salt and sodium hydroxide, followed by calcination of precipitated part at 180°C. The synthesized ZnO NPs properties in terms of phase composition, size and shape were then characterized by X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). To test antibacterial ability of the synthesized ZnO NPs, glass pieces were covered with suspension containing different concentration of ZnO NPs synthesized, afterwards put into Escherichia coli (E.coli) culture after 24 hours of incubation at 37°C. The synthesized ZnO NPs characterized by TEM were mainly rod shape with particles size ranging 30-100 nm; moreover, UV-vis spectrum of the ZnO NPs suspension presented an absorbance peak at 371nm. Furthermore, antibacterial activity of them assessed throughout visualization of E. coli live was found to be influenced on dosed used.