Point-of-Care Chemiluminescence Immunoassay Centrifugal Microfluidics for Gastrin-17 Detection

Gastric cancer is the one of the most common malignant disease worldwide that causes death. Serum gastric17 (G-17) is considered to be a serological marker of gastric cancer. The difficulty of treatment is strongly depended on discover time. It is very important to develop a fast, accurate, low-cost and portable early diagnosis method. The lab-on-adisc (LOAD) or centrifugal microfluidics platform introduces the centrifugal force generated by chip rotation under the driving of micromotor. LOAD is pump-free, providing the best way for multiple parallel operations, which is a good choice for early screening of gastric cancer. In this paper, we present a fully automated lab-on-a-disc for simultaneous detection of G-17 from whole blood based on magnetic enzyme chemiluminescence immunoassay. Serum extraction, metering different concentration ratio and fitting of standard curve can be realized on disc. There is a good linear correlation between chemiluminescence signal intensity and G-17 concentration over the entire measurement range (0-256pmol/L), and the detection limit is 2.11 pmol/L, which has great potential for point-of-care (POC) with high sensitivity and good repeatability.