Extraction Method of Cell’s Complex Permittivity in Cell Solutions From Measured Impedance by GHz Electrical Impedance Spectroscopy

This paper has proposed a new dielectric properties extraction method of cell's complex permittivity ε*
_cell
even in cell solutions from the measured impedance Z*
_sol,
exp
by GHz electrical impedance spectroscopy (EIS) in the frequency range from 10 MHz to 3 GHz. This new extraction method is composed of the electrical impedance measurement of cell solutions and dispersed medium and the dielectric properties calculation based on a combination of plane wave and transmission line theories. By comparing with ε*
_cell,
BH
obtained by Bruggeman-Hanai equation, this new method is proved to extract the complex permittivity of cells ε*
_cell
in cell solutions success fully from the experimental impedances of yeast cell solutions Z*
_sol,
exp
and dispersed medium Z*
_dis,
exp
by GHz EIS with the relative error less than 10% in the frequency range from 10 MHz to 3 GHz. Moreover, the conductivity of cell σ
_cell
among different concentrations obtained by this method shows the smaller discrepancy than that obtained by Bruggeman-Hanai equation in the frequency range from 1 GHz to 3 GHz. The dielectric properties ε*
_cell
and ε*
_cell,
BH
are brought into a simulation model, respectively. The simulated impedance Z*
_sol,
sim
shows a better agreement with the experimental impedance Z*
_sol,
exp
compared with the simulation impedance Z*
_sol,
sim,
BH
, which means the dielectric properties ε*
_cell
obtained by this new method is accurate for the future research of cell's dielectric properties in the GHz frequency range.